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dot blot based proteome profiler human xl cytokine antibody array kit  (R&D Systems)


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    R&D Systems dot blot based proteome profiler human xl cytokine antibody array kit
    Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the <t>cytokine</t> array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.
    Dot Blot Based Proteome Profiler Human Xl Cytokine Antibody Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dot+blot+array+kit/Proteome+Profiler+Human+XL+Cytokine+Array+Kit/pm40492327-92-10-20
    Average 96 stars, based on 526 article reviews
    dot blot based proteome profiler human xl cytokine antibody array kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models."

    Article Title: Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models.

    Journal: Biomaterials science

    doi: 10.1039/d4bm01569j

    Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.
    Figure Legend Snippet: Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

    Techniques Used:

    Related Articles

    Expressing:

    Article Title: Effect of low-level CO 2 on innate inflammatory protein response to organic dust from swine confinement barns
    Article Snippet: Cytokine and chemokine quantitation of BAL fluid was done by enzyme linked immunoabsorbant assay kits to IL-6, KC, and CCL9 (R&D Systems, Minneapolis, MN) according to manufacturer’s instructions. .. Broad spectrum testing of BAL fluid for chemokine expression was accomplished using a dot blot array kit (ARY020, R&D Systems, Minneapolis, MN). ..

    Dot Blot:

    Article Title: Effect of low-level CO 2 on innate inflammatory protein response to organic dust from swine confinement barns
    Article Snippet: Cytokine and chemokine quantitation of BAL fluid was done by enzyme linked immunoabsorbant assay kits to IL-6, KC, and CCL9 (R&D Systems, Minneapolis, MN) according to manufacturer’s instructions. .. Broad spectrum testing of BAL fluid for chemokine expression was accomplished using a dot blot array kit (ARY020, R&D Systems, Minneapolis, MN). ..



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    Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the <t>cytokine</t> array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.
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    A&B. Western blot analysis showing the effect of TET, TRAIL and combination effect of TET with TRAIL on cleaved caspases (caspase-8 and 3) in scrl (Fig. 4A) and DK cells (Fig. 4B). Different concentrations of TET (10–20µM) and TRAIL (80ng/ml) and combinations of TET and TRAIL were used for 24h and 48h. C&D. <t>Apoptosis</t> assay showing the effect of TET, TRAIL and combination treatment of TET and TRAIL on cell death in LNCaP scrl (Fig. 4C) and DK cells (Fig. 4D) at 48h. E. Representative graph showing the percent live and apoptotic scrl and DK cells after TET, TRAIL and combination treatment of TET and TRAIL at 48h. Averages are representative of 3 independent experiments. Error bars represent standard deviation. *p value <0.05; **p value <0.01; ***p value <0.001, ****p value <0.0001.
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    Image Search Results


    Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

    Journal: Biomaterials science

    Article Title: Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models.

    doi: 10.1039/d4bm01569j

    Figure Lengend Snippet: Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

    Article Snippet: Cytokine profiling of the EV groups was performed using the dot blot-based Proteome Profiler Human XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions.

    Techniques:

    A&B. Western blot analysis showing the effect of TET, TRAIL and combination effect of TET with TRAIL on cleaved caspases (caspase-8 and 3) in scrl (Fig. 4A) and DK cells (Fig. 4B). Different concentrations of TET (10–20µM) and TRAIL (80ng/ml) and combinations of TET and TRAIL were used for 24h and 48h. C&D. Apoptosis assay showing the effect of TET, TRAIL and combination treatment of TET and TRAIL on cell death in LNCaP scrl (Fig. 4C) and DK cells (Fig. 4D) at 48h. E. Representative graph showing the percent live and apoptotic scrl and DK cells after TET, TRAIL and combination treatment of TET and TRAIL at 48h. Averages are representative of 3 independent experiments. Error bars represent standard deviation. *p value <0.05; **p value <0.01; ***p value <0.001, ****p value <0.0001.

    Journal: Molecular cancer therapeutics

    Article Title: Tetrandrine (TET) Induces Death Receptors: Apo Trail R1 (DR4) and Apo Trail R2 (DR5) and Sensitizes Prostate Cancer Cells to TRAIL Induced Apoptosis

    doi: 10.1158/1535-7163.MCT-17-1157

    Figure Lengend Snippet: A&B. Western blot analysis showing the effect of TET, TRAIL and combination effect of TET with TRAIL on cleaved caspases (caspase-8 and 3) in scrl (Fig. 4A) and DK cells (Fig. 4B). Different concentrations of TET (10–20µM) and TRAIL (80ng/ml) and combinations of TET and TRAIL were used for 24h and 48h. C&D. Apoptosis assay showing the effect of TET, TRAIL and combination treatment of TET and TRAIL on cell death in LNCaP scrl (Fig. 4C) and DK cells (Fig. 4D) at 48h. E. Representative graph showing the percent live and apoptotic scrl and DK cells after TET, TRAIL and combination treatment of TET and TRAIL at 48h. Averages are representative of 3 independent experiments. Error bars represent standard deviation. *p value <0.05; **p value <0.01; ***p value <0.001, ****p value <0.0001.

    Article Snippet: Dot Blot Array Proteome ProfilerTM Human Apoptosis Array Kit, catalog no. ARY009, (R&D systems, Minneapolis, MN) was used to detect apoptosis-related proteins.

    Techniques: Western Blot, Apoptosis Assay, Standard Deviation

    PCa cells survive because of absence of death receptors hence no apoptosis takes place. These cells develop resistance to TRAIL therefore TRAIL is unable to induce cell death. Upon TET treatment, there is an increase in DR4 and DR5 mRNA and protein levels which cause apoptosis and further treatment of PCa cells with TRAIL sensitizes these cells to TRAIL which enhances activation of caspases and massive cell death.

    Journal: Molecular cancer therapeutics

    Article Title: Tetrandrine (TET) Induces Death Receptors: Apo Trail R1 (DR4) and Apo Trail R2 (DR5) and Sensitizes Prostate Cancer Cells to TRAIL Induced Apoptosis

    doi: 10.1158/1535-7163.MCT-17-1157

    Figure Lengend Snippet: PCa cells survive because of absence of death receptors hence no apoptosis takes place. These cells develop resistance to TRAIL therefore TRAIL is unable to induce cell death. Upon TET treatment, there is an increase in DR4 and DR5 mRNA and protein levels which cause apoptosis and further treatment of PCa cells with TRAIL sensitizes these cells to TRAIL which enhances activation of caspases and massive cell death.

    Article Snippet: Dot Blot Array Proteome ProfilerTM Human Apoptosis Array Kit, catalog no. ARY009, (R&D systems, Minneapolis, MN) was used to detect apoptosis-related proteins.

    Techniques: Activation Assay